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Job ID: 144887

Staff Research Associate I (9613C) - 9217
University of California Berkeley

Date Posted Jul. 27, 2020
Title Staff Research Associate I (9613C) - 9217
University University of California Berkeley
Berkeley, CA, United States
Application Deadline Open until filled
Position Start Date Available immediately
  • Professional Staff
  • Research/Technical/Laboratory

Staff Research Associate I (9613C) - 9217

About Berkeley

At the University of California, Berkeley, we are committed to creating a community that fosters equity of experience and opportunity, and ensures that students, faculty, and staff of all backgrounds feel safe, welcome and included. Our culture of openness, freedom and belonging make it a special place for students, faculty and staff.

The University of California, Berkeley, is one of the world's leading institutions of higher education, distinguished by its combination of internationally recognized academic and research excellence; the transformative opportunity it provides to a large and diverse student body; its public mission and commitment to equity and social justice; and its roots in the California experience, animated by such values as innovation, questioning the status quo, and respect for the environment and nature. Since its founding in 1868, Berkeley has fueled a perpetual renaissance, generating unparalleled intellectual, economic and social value in California, the United States and the world.

We are looking for equity-minded applicants who represent the full diversity of California and who demonstrate a sensitivity to and understanding of the diverse academic, socioeconomic, cultural, disability, gender identity, sexual orientation, and ethnic backgrounds present in our community. When you join the team at Berkeley, you can expect to be part of an inclusive, innovative and equity-focused community that approaches higher education as a matter of social justice that requires broad collaboration among faculty, staff, students and community partners. In deciding whether to apply for a position at Berkeley, you are strongly encouraged to consider whether your values align with our Guiding Values and Principles, our Principles of Community, and our Strategic Plan.

Application Review Date

The First Review Date for this job is: August 6, 2020

Departmental Overview

The Raulet Laboratory investigates mechanisms of recognition of cancer cells and infected cells by natural killer cells and T cells. Furthermore, the Lab investigates mechanisms by which tumors and infectious agents naturally activate or inhibit productive responses by cytotoxic cells, or desensitize the cells by inducing anergy.

The Barton Laboratory studies innate immunity with the goal of understanding strategies of pathogen recognition and self/non-self discrimination. The Lab focuses on the function and regulation of Toll-like receptors (TLRs). TLRs are the prototypical innate immune receptor family. They participate in innate immunity, adaptive immunity, and, in some instances, autoimmunity.

The applicant will work on a unique, joint project which aims to reduce the immunogenicity of the gene editor, Cas9. The first approach is to induce tolerance to Cas9 protein. The second approach is to reduce the immunogenicity of Cas9 by mutating immunodominant epitopes recognized by T cells. Responsibilities are limited to carrying out and evaluating experimental assays and analyzing data.


Under the supervision of principal investigator, perform experiments to address the function of T cells in immune responses. These studies will employ standard immunology and recombinant DNA techniques including:
  • Assays of T cell immune responses in vitro.
  • Immunizations of treatments of mice to induce immune responses or tolerance.
  • Management of mouse breeding colonies.
  • Fluorescence activated cell sorting and analysis.
  • Lymphocyte isolation and cultures.
  • Lymphocyte functional assays.
  • Antibody purifications.
  • ELISAs.
  • Assembly of DNA constructs using recombinant DNA techniques.
  • Transductions and transfections of cell lines or cell populations.
  • PCR analyses, for genotyping and other purposes.

General Lab Maintenance:
  • General bookkeeping and organize laboratory data sets.
  • Perform simple equipment repair and maintenance.
  • Produce and inventory stock solutions.
  • Other duties as assigned.

Required Qualifications
  • Demonstrated knowledge and skills related to the responsibilities of the position
  • Willingness to learn new techniques and skills is essential.
  • Good interpersonal skills are also necessary, as the job will require working in a group environment.
  • Reliability and responsible conduct is essential.
  • B.S. degree in Biology/related field and/or equivalent training/experience.

Preferred Qualifications
  • Familiarity with immunology procedures preferred.
  • Experience with animal handling and husbandry preferred.

Salary & Benefits

This position is a non-exempt, bi-weekly paid position. Hourly pay for this role is commensurate with experience.

For information on the comprehensive benefits package offered by the University visit:

How to Apply

Please submit your cover letter and resume as a single attachment when applying.

Other Information

This position is governed by the terms and conditions in the agreement for the Research Support Professionals Unit (RX) between the University of California and the University Professional and Technical Employees (UPTE). The current bargaining agreement manual can be found at:

Equal Employment Opportunity

The University of California is an Equal Opportunity/Affirmative Action Employer. All qualified applicants will receive consideration for employment without regard to race, color, religion, sex, sexual orientation, gender identity, national origin, disability, or protected veteran status. For more information about your rights as an applicant see:
For the complete University of California nondiscrimination and affirmative action policy see:

To apply, visit


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