Staff Research Associate (9613C) - 9311
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We are looking for equity-minded applicants who represent the full diversity of California and who demonstrate a sensitivity to and understanding of the diverse academic, socioeconomic, cultural, disability, gender identity, sexual orientation, and ethnic backgrounds present in our community. When you join the team at Berkeley, you can expect to be part of an inclusive, innovative and equity-focused community that approaches higher education as a matter of social justice that requires broad collaboration among faculty, staff, students and community partners. In deciding whether to apply for a position at Berkeley, you are strongly encouraged to consider whether your values align with our Guiding Values and Principles
, our Principles of Community
, and our Strategic Plan.
Application Review Date
The First Review Date for this job is: August 12, 2020
The Department of Molecular & Cell Biology (MCB) is home to Undergraduate and Graduate academic programs as well as Faculty Research performed by faculty organized in five divisions:
- Biochemistry, Biophysics and Structural Biology
- Cell and Developmental Biology
- Genetics, Genomics and Development
- Immunology and Pathogenesis
We are looking for a Staff Research Associate to investigate genes critical to the function of the T regulatory immune cell population in disease pathology, including autoimmunity and cancer.
- Mouse colony management - determine what alleles must be genotyped for new mouse litters, harvest tail or blood samples from mice, isolate DNA from tissues, genotype the mice by PCR or flow cytometry, critically assess the results, prediction of genotype frequencies using rules of Mendelian genetics, update mouse genotype database. Capable of designing, testing and optimizing new primers for PCR genotyping of specific alleles. Able to determine antibody stains needed to determine genotype of mice by flow cytometric analysis. Experience injecting mice by sub cutaneous and intra peritoneal injection. Drug delivery by oral gavage. Sub cutaneous tumor inoculations and monitoring by measurement with calipers. EAE induction and monitoring of clinical score. Glucose monitoring for diabetes incidence.
- Cell biological experiments - Harvest tissues from mice and generate single cell suspensions (from lymph nodes, spleen, thymus, tumors, tisssues). Culture of cells using sterile technique counting and propagating cells. Transfection of cells, viral infection of cells, antibody staining of cell surface and intracellular antigens and analysis of cells using FACSCalibur or LSRll flow cytometers. Experience sorting cells by FACS (i.e. use of FACSAria or equivalent).
- Data analysis/ interpretation - daily record keeping in laboratory notebook (including the generation of written protocols for new assays learned), graphing cumulative data generated interpretation of data and drawing conclusions preparation for presentation at lab meetings. Attendance at weekly lab meetings. Background reading/research necessary to understand projects.
- DNA manipulation/cloning - bacterial transformation, bacterial growth, plasmid purification, restriction enzyme digestion for analytical and cloning purposes, agarose gel purification of restricted bands ligation of DNA fragments. Independently design cloning strategies and troubleshoot cloning projects including the capacity to validate proper cloning.
- Demonstrated record of organization, clear communication and excellent attendance.
- Ability to follow instructions precisely.
- Ability to reproducibly perform precision procedures.
- Ability to work independently after training being in research procedures and/or reading assigned literature and standard operating procedures
- Ability to analyze experiments and critique results
- Ability to work collaboratively, learning and sharing techniques and knowledge with other members of the research team.
- Ability to organize and perform several experiments in parallel on the same day.
- Live mouse handling and manipulation (ie injections, bleeding, tagging).
- Mouse necropsy and tissue harvesting.
- Data analysis using software such as flowjo, Microsoft excel (or equivalent).
- Molecular biology techniques including PCR and primer design, cloning by restriction digests and ligation of DNA plasmids agarose gel electrophoresis.
- Cell culture techniques including sterile technique, media preparation, cell growth, transfection, retroviral production, and viral infection.
- Bachelor's degree in Biology or a related science and/or an equivalent combination of education and experience.
- Theoretical knowledge of immunology.
- Research experience in a research lab (academic or industrial).
- Experience organizing and determining mouse genotypes using mendelian genetics, PCR, and flow cytometry.
- Immune cell preparation from mouse blood or lymphoid tissues.
- Antibody staining and flow cytometric analysis using FACSCalibur or LSRII instruments (experienced setting up these machines).
Salary & Benefits
This position is a non-exempt, bi-weekly paid position. Hourly pay for this role is commensurate with experience within the range of $23.34 - $26.01, Steps 1.0 - 6.0.
For information on the comprehensive benefits package offered by the University visit:
How to Apply
Please submit your cover letter and resume as a single attachment when applying.
This position is governed by the terms and conditions in the agreement for the Research Support Professionals Unit (RX) between the University of California and the University Professional and Technical Employees (UPTE). The current bargaining agreement manual can be found at: http://ucnet.universityofcalifornia.edu/labor/bargaining-units/rx/index.html
Conviction History Background
This is a designated position requiring fingerprinting and a background check due to the nature of the job responsibilities. Berkeley does hire people with conviction histories and reviews information received in the context of the job responsibilities. The University reserves the right to make employment contingent upon successful completion of the background check.
Equal Employment Opportunity
The University of California is an Equal Opportunity/Affirmative Action Employer. All qualified applicants will receive consideration for employment without regard to race, color, religion, sex, sexual orientation, gender identity, national origin, disability, or protected veteran status. For more information about your rights as an applicant see:
For the complete University of California nondiscrimination and affirmative action policy see:
To apply, visit https://careerspub.universityofcalifornia.edu/psp/ucb/EMPLOYEE/HRMS/c/HRS_HRAM.HRS_APP_SCHJOB.GBL?Page=HRS_APP_JBPST&Action=U&FOCUS=Applicant&SiteId=21&JobOpeningId=9311&PostingSeq=1